After incubation, the bacteria appear to be growing throughout the tube. FACTORS INFLUENCING THE GROWTH OF MICROBES . Growth factors. 2. Just growing bacteria in a Petri dish is not a science fair experiment. 2. Factors Affecting Microbial Growth The key to a productive staff is creating and maintaining a safe and healthy work environment. Factors That Affect Transformation Efficiency. Bacterial transformation is a common technique in molecular biology that every student of the life sciences should be familiar with. Maintaining work equipment and the physical state of a building is important, but there are potential threats lurking in a company’s infrastructure that are too small for the naked eye to see. Temperature requirement. Before we go over these factors we must however understand the bacterial growth curve. The Effect of Alkalines and Acids on Bacterial Growth Biology Projects,Science Experiments Project Ideas, Science Experiment Ideas, Model Topics, Exhibition Ideas, Expo Activities for CBSE ICSE for Kids and also for Middle school, Elementary School for class 5th Grade,6th,7th,8th,9th 10th,11th, 12th Grade and High School , MSC and College Students. Growth factors are organic compounds such as amino acids , purines , pyrimidines , and vitamins that a cell must have for growth but cannot synthesize itself. Choose an appropriate strategy for this experiment. Laboratory Exercise 14 PHYSICAL AND CHEMICAL FACTORS AFFECTING GROWTH Objectives: At the end of the experiment, the student must be able to: 1. So, we put it up on the window sill to see how it would do with the rest of the glasses. There are many factors that affect bacterial growth. Factors affecting bacterial growth: _____ (Fig 6.17) - formation of endospores in Bacillus, Clostridium and a few other organisms in response to harsh conditions. Endospores are dormant, and are resistant to heat, radiation, drying, and many chemicals. If you want to do a science fair project about germs, you have to add a variable, or something that changes in the experiment. The survival and growth of a microbe is a function of a myriad of different activities: nutrient assimilation, catabolism, and the synthesis of living cytoplasm and all cellular structures. 1) Oxygen requirement: Bacteria are divided into four groups according to oxygen requirement: 1. Transformation is the introduction of foreign DNA into a bacterial cell. ... A bacterium's osmotic environment can affect bacterial growth. Bacteria can be grouped according to their energy source as phototrophs or chemotrophs. A small group of cells are placed in a nutrient rich medium that allows them to synthesize proteins and other molecules necessary for replication. What are environmental factors that affect microbial growth?-atmospheric gases-temperature-pH-osmotic pressure ... A tube of fluid thioglycliate medium is inoculated with a bacterial isolate. Oxygen requirement. Factors That Affect Bacterial Growth . 3. Each of these As E.coli grows in the intestines its optimal temperature is about 37 degrees c.pHE. coli can survive in stomach acid of pH 2 but optimal pH for the growth of E.Coli is… Bacteria grow only in the presence of oxygen, called: strict aerobes. Lag Phase: This initial phase is characterized by cellular activity but not growth. ... Dd had mixed up the different experiments we were to do during the week, and thought one glass was to have yeast added to it. EXPERIMENT 6. TemperatureLow temperatures slow down while temperatures higher than 40 degrees c will denature the bacteria, an optimal temperature is needed for optimal growth. All these various activities are performed by specific enzymes. Determine the effects of several chemical and physical factors on the growth of different microorganisms; 2. Transformation happens very slowly, but … Osmotic pressure (salt tolerance). Occurs in stationary phase, when nutrients are limiting, and/or wastes have accumulated. The bacterial growth curve represents the number of live cells in a bacterial population over a period of time. Factors affecting bacterial growth Factors affecting bacterial growth are: 1. Classify different bacterial species according to their response to various temperature, pH, oxygen and salinity/osmolarity. Yes, it is gross and cool and fascinating, but it doesn’t meet the requirements of a science fair project. PH 4. 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